dylight 649 vector labs cat dl 1068 1 Search Results


dylight 649 labeled uea  (Vector Laboratories)
95
Vector Laboratories dylight 649 labeled uea
Dylight 649 Labeled Uea, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ulex europaeus agglutinin i  (Vector Laboratories)
96
Vector Laboratories ulex europaeus agglutinin i

Ulex Europaeus Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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fluorescein labeled lotus tetragonolobus ltl lectin  (Vector Laboratories)
96
Vector Laboratories fluorescein labeled lotus tetragonolobus ltl lectin
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Fluorescein Labeled Lotus Tetragonolobus Ltl Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dylight+649+vector+labs+cat+dl+1068+1/Fluorescein+labeled+Lotus+Tetragonolobus+Lectin+(LTL)/pmc08620232-47-27-35
Average 96 stars, based on 1 article reviews
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rabbit anti cd31  (Proteintech)
96
Proteintech rabbit anti cd31
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Rabbit Anti Cd31, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti hif 2α  (Bethyl)
93
Bethyl rabbit anti hif 2α
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Rabbit Anti Hif 2α, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti enos  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti enos
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Rabbit Anti Enos, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dylight+649+vector+labs+cat+dl+1068+1/eNOS+Antibody/pm38509349-444-52-53
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mouse anti actb antibody  (Proteintech)
94
Proteintech mouse anti actb antibody
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Mouse Anti Actb Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti gapdh antibody  (Proteintech)
96
Proteintech mouse anti gapdh antibody
Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), <t>LTL</t> (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 <t>lectin</t> (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test
Mouse Anti Gapdh Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dylight+649+vector+labs+cat+dl+1068+1/HRP-conjugated+GAPDH+Monoclonal+antibody/pmc11237007__41467_2024_50223_MOESM1_ESM-86-119-123
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Image Search Results


Journal: iScience

Article Title: Single-cell atlas of the aging mouse colon

doi: 10.1016/j.isci.2022.104202

Figure Lengend Snippet:

Article Snippet: Ulex Europaeus Agglutinin I (UEA-1), Dylight® 649 coupled , Vector Labs , Cat# DL-1068-1.

Techniques: Recombinant, Lysis, Plasmid Preparation, Multiplex Assay, Staining, RNA Sequencing Assay, Sequencing, Real-time Polymerase Chain Reaction, Software, Imaging, Light Microscopy, Microscopy, Inverted Microscopy

Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), LTL (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 lectin (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test

Journal: Respiratory Research

Article Title: Mucus threads from surface goblet cells clear particles from the airways

doi: 10.1186/s12931-021-01898-3

Figure Lengend Snippet: Mucus bundles made by submucosal glands were transported by cilia. A Schematic drawing of the tilted table with heating to 37 °C where pig distal trachea and primary bronchi were mounted to ensure air–liquid interface and transport against gravity. B Image sequence from two sequential low-resolution time-lapses lasting five minutes each. Alcian blue stained mucus bundles (arrows) on explanted trachea from a weaned WT piglet. Speed of corresponding movies (Additional files and ) increased 16×. C Bundle thickness measured on the airway surface, each data point is the mean of at least ten measurements per bundle, median with interquartile range. WT: 3 piglets, 3 time-lapses, 8 bundles. CF: 3 piglets, 4 time-lapses, 11 bundles. D Explanted WT piglet airway mucus bundles stained with Alcian blue (blue), LTL (green) and merged low-resolution image. E Confocal high-resolution image of formalin-fixed paraffin section from a newborn piglet trachea stained with antibodies against MUC5B and MUC5AC, illustrating the production of MUC5B (green) in submucosal mucous cells and mostly MUC5AC (red) in surface goblet cells. Some MUC5B was observed in surface cells. F Surface goblet cell mucus stains with the UEA1 lectin (red), whereas mucus in submucosal glands (MUC5B) stains with the lectin LTL (green). G Explanted WT piglet airway with LTL stained mucus bundles, low-resolution image. Arrowheads indicate gland openings. H Bundle thickness measured on the airway surface compared to at the gland openings. The mean of ten measurements per opening is presented as one data point. Data presented as median with interquartile range. WT: 3 piglets, 9 time-lapses, 36 bundles. CF: 2 piglets, 3 time-lapses, 8 bundles. WT surface vs. WT opening P = 0.0025 **, Kruskal–Wallis and Dunn´s multiple comparisons test

Article Snippet: To visualize the different components of the mucus, tissue was stained with 0.4 mM Alcian blue 8GX pH 7.4 (Cat# A5268, Sigma-Aldrich, St. Louis, MO), 5 µg/ml fluorescein labeled Lotus tetragonolobus (LTL) lectin (Cat# FL-1321-2, Vector laboratories, Burlingame, CA) and/or 5 µg/ml DyLight 649 labeled Ulex europaeus agglutinin (UEA1) (Cat# DL-1068-1, Vector laboratories, Burlingame, CA) dissolved in oxygenated Krebs-glucose buffer, pH 7.4.

Techniques: Sequencing, Staining, Paraffin Section